Pb7wgf2

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August undefined, 2024

Splet03. apr. 2024 · The full AtMiro2 coding sequence was cloned using Gateway reactions (Invitrogen) into the binary destination vector PB7WGF2 creating an N-terminal GFP fusion. Using this as template, the ... Splet23. okt. 2024 · For SlACRE75, SlACRE180, NbACRE75 and NbACRE180, cDNAs from pUC57 entry vector were transformed by electroporation into Escherichia coli strain DH10B and …

IQ67 DOMAIN proteins facilitate preprophase band formation and …

SpletCurrent Biology, Volume 30 Supplemental Information A Plasma Membrane Nanodomain Ensures Signal Speciﬁcity during Osmotic Signaling in Plants Marija Smokvarska, Charbel Francis, Matthieu Pierre Platre, Jean-Bernard Fiche, Carine Splet1.构建10kb大小，具有绿色荧光蛋白的质粒载体pb7wgf2.0； 2.用qiagen公司生产的qiafilter tm plasmidmidikit试剂盒制备质粒dna；设置质粒dna浓度为600ng/μl； 3.用sigma-aldrich … boeing 777-300er seat plan thai

Phytophthora infestans RXLR Effectors Target Parallel Steps in an ...

Splet11. nov. 2015 · Chilling stress is a production constraint of tomato, a tropical origin, chilling-sensitive horticultural crop. The development of chilling tolerant tomato thus has significant potential to impact ... Splet16. okt. 2024 · Agrobacterium tumefaciens carrying plasmids to express mRFP or GFP (empty pB7WGF2, or pB7WGR2 respectively) were infiltrated into the air spaces of one … SpletLOG IN. Minimum purchase order is 150,00€. Free shipping on all products. Leave this field blank. Gateway vectors Log In - pB7WGF2 Gateway Vectors Download .Gb File - pB7WGF2 Gateway Vectors Other Resources In this section, clones and vectors co-developed by the Department … For questions related to payments, MTAs, and technical issues, please contact: … The host strain of all plasmids is E. coli DB3.1 or ccdB survival cells from … global air traffic statistics 2022

Ectopic expression of Arabidopsis glutaredoxin

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Splet20. okt. 2024 · An extract of N. benthamiana leaves expressing GFP alone (pB7WGF2.0) was prepared similarly and served as mock control for treatments. For monitoring hypersensitive responses ... Splet05. jul. 2012 · pB7WGF2/GFP-AtGRXS17 was introduced into Agrobacterium strain GV3101 as described and then used for agro-infiltration as previously described (Liu et al., 2005). For heat shock treatment, at 1.5–2 days postinfiltration (DPI), the infiltrated leaves were detached from tobacco plants, kept in Petri dishes with the moistened filter paper and ... boeing 777-300er seating chart swiss airSplet28. jan. 2024 · Effectors were cloned into pB7WGF2 and the constructs were transformed into Agrobacterium tumefaciens strain GV3101. Agrobacterium cells were harvested by … global alarms orlando

"Splet05. jul. 2012 · pB7WGF2/GFP-AtGRXS17 was introduced into Agrobacterium strain GV3101 as described and then used for agro-infiltration as previously described (Liu et al., 2005). … " - Pb7wgf2

Pb7wgf2

Splet26. sep. 2024 · The construct 35Sp-GFP (pB7WGF2 empty vector) was used as a control. These constructs were introduced into Col-0 or jaz4-1 plants according to the floral-dip method for plant transformation (Clough and Bent, 1998). Overexpresser lines were selected by spraying a 0.0114% glufosinate ammonium (BASTA, Bayer®, Whippany, NJ, … Splet01. jan. 2002 · To further verify our optimised protocol for PPT selection, we transformed BY-2 cells with another plasmid (pB7WGF2_GFP-SCO3) derived from the GATEWAY™ destination vector (Karimi et al. 2002 ...

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Splet19. dec. 2024 · The mutant Stphot1.KD was recombined, using LR clonase (Invitrogen), into pB7WGF2 or pK7WGR2 vectors for in planta expression and in vitro kinase assays. … SpletLOG IN. Minimum purchase order is 150,00€. Free shipping on all products. Leave this field blank. Gateway vectors

Splet24. maj 2024 · The sequences were mobilized into pBGWFS7, pB7FWG2 or pB7WGF2 (ref. 31) to generate promoter:GFP–GUS reporter lines and p35S promoter driven C- and N-terminal translational GFP fusion constructs ... Splet04. jan. 2024 · The coding sequence of GRF4 was cloned into the pSuper1300-MYC vector to construct 35S::GRF4-MYC, and the coding sequence of GRF4 S248A was cloned into the pB7WGF2.0 vector to obtain 35S::GFP-GRF S248A. The S248A mutation was introduced in the GRF4 coding sequence by polymerase chain reaction (PCR), using the primers listed …

SpletpB7WGF2 plasmid vector植物表达载体质粒 BioVector NTCC质粒载体菌种细胞基因保藏中心. 价格： ¥29865. 货号： pB7WGF2 plasmid vector植物表达载体质粒. 产地：北京. 点击 … SpletThermo Fisher pb7wgf2 vector. Pb7wgf2 Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, …

Splet01. jan. 2002 · To further verify our optimised protocol for PPT selection, we transformed BY-2 cells with another plasmid (pB7WGF2_GFP-SCO3) derived from the GATEWAY™ …

SpletLOG IN. Minimum purchase order is 150,00€. Free shipping on all products. Leave this field blank. Gateway vectors global air travel industrySplet26. sep. 2024 · The construct 35Sp-GFP (pB7WGF2 empty vector) was used as a control. These constructs were introduced into Col-0 or jaz4-1 plants according to the floral-dip … boeing 777-300er seating chart turkish airSplet03. avg. 2024 · The pB7WGF2-BPC6 construct was transformed into Agrobacterium tumefaciens strain GV3105 via electroporation. The A. tumefaciens cells that contained pB7WGF2-BPC6 were then introduced into WT. The resulting T1 pB7WGF2-BPC6 transgenic plants were selected by BASTA as described previously (Sun et al., 2016). Homozygous … boeing 777 300er seat map american airlinesSplet23. nov. 2024 · Agrobacterium tumefaciens GV3101 transformed with pB7WGF2.0 or pK7WGR2.0 harboring each gene of interest or empty (as control) were cultivated … boeing 777 300er sitzplan air franceSplet23. okt. 2024 · For SlACRE75, SlACRE180, NbACRE75 and NbACRE180, cDNAs from pUC57 entry vector were transformed by electroporation into Escherichia coli strain DH10B and transferred by a recombinant LR reaction of Gateway cloning (Clonase II enzyme mix Kit, Thermo Fisher) into pB7WGF2 (Karimi et al., 2002). Transient expression in Nicotiana … boeing 777-300er sitzplan air canadaSplet19. jun. 2024 · Agrobacterium tumefaciens containing overexpression vector pB7WGF2 was used to transform leaf discs of N. benthamiana. Positive lines were first screened on … boeing 777-300er seat map air new zealand boeing 777-300er seating chart lufthansa